International Journal of Research and Reports in Hematology http://journalijr2h.com/index.php/IJR2H <p style="text-align: justify;"><strong>International&nbsp;Journal of Research and Reports in Hematology</strong>&nbsp;aims to publish&nbsp;high-quality&nbsp;papers (<a href="/index.php/IJR2H/general-guideline-for-authors">Click here for Types of paper</a>) in all areas of&nbsp;‘Hematology’. This journal facilitates the research and wishes to publish papers as long as they are technically correct, scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN&nbsp;peer-reviewed, open access INTERNATIONAL journal.</p> en-US contact@journalijr2h.com (International Journal of Research and Reports in Hematology) contact@journalijr2h.com (International Journal of Research and Reports in Hematology) Sat, 26 Oct 2019 08:19:03 +0000 OJS 3.1.1.4 http://blogs.law.harvard.edu/tech/rss 60 Bigi Soft Drinks might Induce Hyperglycemia and Hyperlipidemia in Wistar Rats http://journalijr2h.com/index.php/IJR2H/article/view/30111 <p><strong>Background</strong>: Bigi soft drinks are carbonated drinks produced by Rite Foods Limited. The company is an indigenous company in Nigeria. Before 2016, Coca-cola bottling company and 7up bottling company products were the dominant soft drinks in Nigeria. Rite Foods Limited introduced carbonated soft drinks into the Nigerian market in 2016 and have favourably competed with the existing products. As at today, Bigi soft drinks are the dominant soft drinks in Nigeria because of their palatable taste, large volume and low price.</p> <p><strong>Aim</strong>: This study sought to investigate the effect of Bigi soft drinks on fasting blood glucose and lipid profile of Wistar rats.</p> <p><strong>Methods</strong>: Thirty-five adult Wistar rats were used for this study. They were randomly divided into seven groups of five rats each after seven days acclimatization. They were treated accordingly: animals in group 1 were administered distilled water, those in group 2 were administered Bigi Cola, those in group 3 were administered Bigi Apple, those in group 4 were administered Bigi Tropical, those in group 5 were administered Bigi Orange, those in group 6 were administered Bigi Lemon and Lime, while those in group 7 were administered Bigi Chapman. The administration was done orally at a dose of 3 mL per 100 g body weight 12 hourly for fourteen days. At the end of the administration period, the animals were fasted overnight and anaesthetized using diethyl ether. Blood samples were collected by cardiac puncture. Fasting blood glucose and lipid profile were determined using standard methods.</p> <p><strong>Results</strong>: All the soft drinks used in this study (except Bigi Lemon and Lime) significantly increased the fasting blood glucose of animals. All the Bigi soft drinks (except Bigi Cola) significantly increased triglyceride, total cholesterol and VLDL of animals when compared with control at p&lt;0.05 respectively. The soft drinks also perturbed the HDL and LDL of animals used in this study.</p> <p><strong>Conclusion</strong>: The result of this study implies that Bigi soft drinks might be deleterious to health as far as hyperglycemia and hyperlipidaemia is concern. This does not automatically translate to such effect on humans. However, individuals with a diabetic family history should minimize their consumption of these drinks.</p> Augustine I. Airaodion, Emmanuel O. Ogbuagu, John A. Ekenjoku, Victor N. Okoroukwu, Uloaku Ogbuagu ##submission.copyrightStatement## http://journalijr2h.com/index.php/IJR2H/article/view/30111 Sat, 26 Oct 2019 00:00:00 +0000 Assessment of CD34+ Cells and Total Nucleated Cells in Umbilical Cord Blood in a Tertiary Hospital South-south, Nigeria http://journalijr2h.com/index.php/IJR2H/article/view/30112 <p><strong>Aim:</strong> Umbilical cord blood (UCB) contains sufficient number of haematopoietic stem cell and progenitor cells that can be used for autologous and allogeneic stem cell transplantation in children and adolescents. Our study assessed the CD34+ cells and total nucleated cells in umbilical cord blood stem cells in a tertiary institution in Nigeria.</p> <p><strong>Study Design:</strong> This is a cross-sectional study.</p> <p><strong>Place and Duration of Study:</strong> This study was conducted in University of Benin Teaching Hospital (UBTH), Benin City. Informed consent for UCB collection was obtained from healthy mothers with uncomplicated pregnancies, receiving care at the Department of Obstetrics between July and September, 2016.</p> <p><strong>Methodology:</strong> A total of forty umbilical cord bloods samples were collected from the placenta umbilical cord after delivery. CD34+ cells were enumerated using flow cytometer while haematology analyzer was used to assess total nucleated cell (TNC) count. Data was analysed using Statistical Package for Social Sciences (SPSS) version 21.</p> <p><strong>Results: </strong>CD 34+ cells count ranged between 2.0 - 6.99 x 10<sup>4</sup>cells/ml with a mean value of 3.89 ± 1.48 x 10<sup>4</sup>cells/ml (Recommended minimum value 2x10<sup>5</sup>/kg). Mean value of TNC was 11.14 ± 4.47 x 10<sup>6</sup> cells/ml with a range of 4.80-21.10 x10<sup>6 </sup>cells/ml (Recommended minimum value 2x 10<sup>7</sup> /kg). We observed a positive correlation between CD34+ cells and TNC count (r = 0.760, p=0.000).&nbsp; In addition, maternal parity showed a significant inverse relationship with TNC and CD34+ cells.</p> <p><strong>Conclusion: </strong>CD34+cells and TNC count of UCB obtained from placentae of babies delivered at the University of Benin Teaching Hospital are within the acceptable values for haematopoietic stem cell transplantation. This is in keeping with recommendations by the World Marrow Donor Association, which stated that a minimum of 2 x10<sup>7 </sup>TNC/kg or 2 x10<sup>5</sup> CD34+ cells/kg of body weight of recipient.</p> Matilda Adesuwa O. Ojo, Tomisin Matthew Adaja, Oluwafemi Adeyemi, Patrick Olanrewaju Osho, Ehigha Enabudoso, Godwin Nosakhare Bazuaye ##submission.copyrightStatement## http://journalijr2h.com/index.php/IJR2H/article/view/30112 Mon, 28 Oct 2019 00:00:00 +0000