Association of Haptoglobin Gene Polymorphism with Uncomplicated Malaria Cases in Elfasher, Northern Darfur State, Sudan
International Journal of Research and Reports in Hematology,
Page 10-19
Abstract
Background: Malaria is an acute febrile illness caused by Plasmodium parasites. Malaria was one of the major problems in Sudan, recently the emergence of malaria in Northern Darfur lead to the outbreak in the 2018 and 2019 high morbidity and mortality, the host parasites interaction may be one the reason.
Haptoglobin is an acute phase protein that binds haemoglobin, preventing iron loss and renal damage. It also serves as an antioxidant, has antibacterial activity, and modulates many aspects of the acute phase response.
Methodology: To determine polymorphism in Haptoglobin genes in malaria patients, using polymerase chain reaction (PCR) in Elfasher city Northern Darfur State, durning the transmission season of August 2020 to December 2021.
A total of 142 individuals were included, 26.8% males and 73.2%females. (Data variables were demographic, clinical, parasitological and some hematological parameters), were reported using chi square test. Comparisons of continuous variables using the one-way analysis of variance ANOVA for parametric data and Kruskal Wallis test for non-parametric data. An alpha value of < 0.05 denoted a statistically significant difference in all statistical comparisms.
Results: Association of Hp genotypes and the risk of uncomplicated malaria were analyzed by age and gender. Hp1-1 genotype was most frequency (44.4%) compared to other genotypes. The Hp1-1, Hp2-1 and Hp2-2 genotypes were found in 88.9%%, 100% and 71.1% of female patients, respectively. None of age and gender factors revealed statistically significant association (P = 0.05).
The Hp2-2 genotype was higher with 89.2% on malaria patients with fever compared to other genotypes. It is possible that the Hp2-2 genotype protects against a range of malaria symptoms, but we did not find a significant association between Hp genotypes and malaria clinical symptoms (P = 0.05).
Conclusion: This research revealed that Hp 1–1 and Hp 2–1 genotypes each occur in nearly 4 in 10 children and the Hp 2–2 genotype occurs in 2 of 10 children. There was no correlation with the prevalence of uncomplicated malaria. To fully comprehend the significance of these genotypes in malaria protection, more research on the effects of haptoglobin genotypes on the severity of P. falciparum malaria is required.
Keywords:
- Haptoglobin
- polymorphism
- uncompleted malaria
- Elfasher
How to Cite
References
[Google Scholar]
Monroe A, Williams N, Ogoma S, Karema C, Okumu F. Reflections on the 2021 World Malaria Report and the future of malaria control. Malaria Journal. 2022; 21(1).
Wassell J. Haptoglobin: function and polymorphism. Clin Lab. 2000;46(11-12):547-52.
PMID: 11109501.
Kathleen M. Madden, Jay S. Raval, Chapter 4 - Allergic Transfusion Reactions, Editor(s): Robert W. Maitta, Immunologic Concepts in Transfusion Medicine, Elsevier. 2020;45-54.
Available:https://doi.org/10.1016/B978-0-323-67509-3.00004-4
Ivan T. Drvenica, Ana Z. Stance’s, Irina S. Maslovar, Drenka Trivanov, Vesna. Biomolecules 2022;12:1708.
Available:https://doi.org/10.3390/biom12111708
Federal Ministry of Health. Sudan Malaria Treatment Protocol, Republic of Sudan, 17–18. Federal Ministry of Health; 2017.
Abossie A, Yohanes T, Nedu A, Tafesse W, Damitie M. Prevalence of malaria and associated risk factors among febrile children under five years: A cross-sectional study in Arba Minch Zuria District, South Ethiopia. Infect Drug Resist. 2020;13: 363-372.
DOI: 10.2147/IDR.S223873. PMID: 32104008; PMCID: PMC7012238.
Seble Aregawi, Lei Li and Caterina M. Miraglia. Malaria rapid diagnostic test and Giemsa – stained peripheral blood smear discrepancies in the diagnosis of Plasmodium ovale infection in New England. American Society for Clinical Laboratory Science. 2017;30(2): 75-83.
DOI: https://doi.org/10.29074/ascls.30.2.75
Wan BN, et al. Progress on haptoglobin and metabolic diseases. World journal of diabetes, 2021;12(3):206-214.
Banini BA, Cazanave SC, Yates KP, Asgharpour A, Vincent R, Mirshahi F .Nonalcoholic Steatohepatitis Clinical Research Network (NASH CRN). Haptoglobin 2 Allele is Associated With Histologic Response to Vitamin E in Subjects With Nonalcoholic Steatohepatitis. J Clin Gastroenterol. 2019; 53(10):750-758.
DOI: 10.1097/MCG.0000000000001142. PMID: 30586008; PMCID: PMC6588507.
Oluwagbenga Ogunfowokan, Bamidele A. Ogunfowokan and Anthony I. Nwajei. Sensitivity and specificity of malaria rapid diagnostic test (mRDT CareStatTM) compared with microscopy amongst under five children attending a primary care clinic in southern Nigeria. African Journal of Primary Health Care & Family Medicine; 2020.
Available:https://hdl.handle.net/10520/EJC-1eae8887ea
Dokunmu TM, Obi PO, Fatiregun OA, Rotimi OA, Agodirin SO, Rotimi SO. Haptoglobin genotypes and malaria comorbidity in breast cancer and healthy Nigerian women. Ann Afr Med. 2022; 21(3):231-236.
DOI: 10.4103/1596-3519.356811. PMID: 36204908; PMCID: PMC9671171.
Osman NM, Elseed HAG, Elagib AA. Study of haptoglobin phenotypes in sudanese diabetic patients. American Journal of Microbiological Research. 2016;4(2):61-63.
Lwanira CN, Kironde F, Swedberg G. Haptoglobin gene diversity and incidence of uncomplicated malaria among children in Iganga, Uganda. Malar J. 2020;19:435.
Available:https://doi.org/10.1186/s12936-020-03515-y
Penha-Gonçalves C. Genetics of malaria inflammatory responses: A pathogenesis perspective. Front. Immunol. 2019; 10:1771.
DOI: 10.3389/fimmu.2019.01771
Lwanira CN, Kironde F, Swedberg G. Haptoglobin gene diversity and incidence of uncomplicated malaria among children in Iganga, Uganda. Malar J. 2020;19:435.
Available:https://doi.org/10.1186/s12936-020-03515-y
Somer S, Levy AP. The role of haptoglobin polymorphism in cardiovascular disease in the setting of diabetes. Int. J. Mol. Sci. 2021;22:287.
Available:https://doi.org/10.3390/ijms22010287
Kengne Fotsing CB, Pieme CA, Biapa Nya PC, Chedjou JP, Dabou S, Nguemeni C, Teto G, Mbacham WF, Gatsing D. Relation between haptoglobin polymorphism and oxidative stress status, lipid profile, and cardiovascular risk in sickle cell anemia patients. Health Sci Rep. 2022;5(1): e465.
DOI: 10.1002/hsr2.465. PMID: 35229039; PMCID: PMC8865067.
Merkler A, Sertić J, Bazina Martinović A, Križ T, Militia I, Šimić M, Caban D, Ljubić H, Markeljević J, Šimičević L, Kaštelan S, Pećin I, Reiner Ž. Haptoglobin genotype 2-2 associated with atherosclerosis in patients with ischemic stroke. Gene. 2020;752: 144786.
DOI: 10.1016/j.gene.2020.144786. Epub 2020 May 18. PMID: 32439379.
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