Hepatoprotective and Hematological Effect of Aqueous Extract of Bombax costatum in Wistar Rat

Elendu Melford Uche *

Department of Human Physiology, Faculty of Basic Medical Sciences, Imo State University, Owerri, Imo State, Nigeria.

Ejiofor Dominic Chinedu

Department of Human Physiology, Faculty of Basic Medical Sciences, Imo State University, Owerri, Imo State, Nigeria.

Ogwo Elisha Uko

Department of Human Physiology, Faculty of Basic Medical Sciences, Abia State University, Uturu, Abia State, Nigeria.

Ubani Clement Dimgba

Department of Human Physiology, Faculty of Basic Medical Sciences, Abia State University, Uturu, Abia State, Nigeria.

Azunna Uchenna

Department of Human Physiology, Faculty of Basic Medical Sciences, Abia State University, Uturu, Abia State, Nigeria.

*Author to whom correspondence should be addressed.


Abstract

The aim of this study was to evaluate the hepatoprotective and hematological effects of aqueous extract of Bombax costatum in Wistar rats. Freshly harvested leaves of Bombax costatum washed with clean tap water were air dried at room temperature. The dried leaves were subsequently ground into fine powder with the aid of an electric blender. Exactly 500 g of powdered plant sample was processed into extract. Thirty (30) adult male Wistar rats were divided into six groups of five rats each. Group I was the normal control administered 2 ml of distilled water. Group II was the negative control i.e., induced liver damage without treatment. Groups III-V were administered 100, 200 and 400 mg/kg of extract of B. constatum respectively prior to induction of hepatic damage. Treatment lasted for 10 days after which animals were sacrificed and blood sample collected. Qualitative phytochemical screening, biochemical and hematological parameters were performed using standard procedures. While anthraquinone was reportedly absent, saponin was the most abundant of the phytochemicals reportedly present. The activity of the liver enzymes was high in the negative control which was induced hepatic damage without treatment. However, it was significantly (P<0.05) lower in groups which were pre-treated with aqueous extract of Bombax constatum prior to induction attempt. It was also observed that Packed Cell Volume (PCV), Hemoglobin concentration (Hb) and Red Blood Cell (RBC) reported for the negative control were significantly (P<0.05) lower than that reported for the normal control. However, in groups which were administered with the said extract before induction attempt, PCV, Hb, and RBC were significantly (P<0.05) higher than those reported for group II which were induced hepatic damage without treatment.  In conclusion, aqueous extract of B. costatum has hepato and hemato protective effects.

Keywords: Liver, phytochemicals, bombax costatum, blood, hemoglobin


How to Cite

Uche, Elendu Melford, Ejiofor Dominic Chinedu, Ogwo Elisha Uko, Ubani Clement Dimgba, and Azunna Uchenna. 2024. “Hepatoprotective and Hematological Effect of Aqueous Extract of Bombax Costatum in Wistar Rat”. International Journal of Research and Reports in Hematology 7 (1):33-38. https://journalijr2h.com/index.php/IJR2H/article/view/139.

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References

Ozougwu JC, Eyo JE. Hepatoprotective effects of Allium cepa extracts on paracetamol-induced liver damage in rat. African Journal of Biotechnology. 2014;13 (26):2679 -2688.

Ozougwu JC. Comparative hepatoprotective and antioxidant effects of Allium cepa, Allium sativum and Zingiber officinale methanolic extracts against paracetamol-induced liver damage in Rattus novergicus. Ph.D Research Thesis, Department Of Zoology and Environmental Biology, University of Nigeria, Nsukka. 2014;222.

Bing G, Yuming G, Qucuo N, Yuemei F, Ziyun W, Rong L et al. Exposure to air pollution is associated with an increased risk of metabolic dysfunction-associated fatty liver disease. Journal of Hepatology. 2022;76 j 518–525.

Burkill HM. The useful plants of West Tropical Africa. 2nd Edition. 1985;1.

Cook JA, VanderJagt DJ, Dasgupta A, Mounkaila G, Glew RS, Blackwell W, et al: Use of the Trolox assay to estimate the antioxidant content of seventeen edible wild plants of Niger. Life Sci. 1998;63(2):105-110.

Zimmerman M. Ethical guidelines for investigations of experimental pain in conscious animals. Pain. 1983;16(2):109-110.

Harbourne JB. Phytochemical methods: A guide to modern technique of plant analysis. 2nd edition London: Chapman and Hall Ltd. 1998;282.

Lorke D. A new approach to practical acute toxicity testing. Arch Toxicol. 1983;54 (4):275-287.

Yang M, Wu Z, Wang Y, Kai G, Singor Njateng GS, Cai S et al. Acute and subacute toxicity evaluation of ethanol extract from aerial parts of Epigynum auritum in mice. Food Chemical Toxicology. 2019;131(1):110534.

Bassey OA, Lowry OH, Brock MJ. A method for the rapid determination of alkaline phosphates with five cubic millimetres of serum. Journal of Biological Chemistry. 1946;164(1):321-325.

Wright PJ, Leatherwood PD, Plummer DT. Enzymes in rats: Alkaline phosphatase. Enzymologia. 1972;42(1):317-327.

IFCC (International Federation of Clinical Chemistry) Physicochemical quantities and units in clinical chemistry. Journal of Clinical Chemistry and Clinical Biochemistry. 1980;18(1):829-854.

Osadebe PO, Okoye FB, Uzor PF et al. Phytochemical analysis, hepatoprotective and antioxidant activity of Alchornea cordifolia methanol leaf extract on carbon tetrachloride-induced hepatic damage in rats. Asian Pac J Trop Med. 2012;5:289-293.

Jha M, Nema N, Shakya K, Ganesh N, Sharma V. In vitro hepatoprotective activity of Bauhinia variegata. Pharma-cologyonline. 2009;3:114-118

Gupta P, Goyal R, Chauhan Y, Sharma PL. Possible modulation of FAS and PTP-1B signaling in ameliorative potential of Bombax Ceiba against high fat diet induced obesity, Complement. Altern. Med. 2013;13:281–289.

Arafaa AF, Foda DS, Mahmoud AH, Metwally NS. ARH Farrag Bombax ceiba flowers extract ameliorates hepatosteatosis induced by ethanol and relatively moderate fat diet in rats. Toxicology Reports. 2019;6:401-408.

Mukharjee JB. Forensic medicine and toxicology (2nd Edn). Families A–D. Royal Botanic Gardens, Kew, Richmond, United Kingdom. 2000;960.

Asgary S. Protective effect of flavonoids against red blood cell hemolysis by free radicals. Exp Clin Cardiol. 2005; 10(2).