Consumption of Tiger Nut (Cyperus esculentus L.) Improves Haematopoiesis in Wistar Rats

Main Article Content

Augustine Ikhueoya Airaodion
Emmanuel Onyebuchi Ogbuagu


Background: The consumption of tiger nut has often been relegated only to the poor due to its affordability.

Aim: This study is therefore aimed at assessing its effect on haematological parameters in Wistar rats.

Materials and Methods: Twenty-four adult Wistar rats were divided into four groups of six each. Animals in group A were administered normal saline solution while those in groups B, C and D were administered 1, 2 and 4 mL/kg body weight of undiluted tiger nut  milk respectively for twenty-eight days. The administration was done 12 hourly via the oral route. At the end of the administration, the rats were sacrificed after an overnight fast under diethyl ether as anesthesia. Blood samples were collected via cardiac puncture. Haematological parameters were determined using standard methods.

Results: A dose-dependent increase was observed in packed cell volume (PCV), haemoglobin (Hb), red blood cell (RBC) and mean corpuscular haemoglobin concentration (MCHC) of animals treated with tiger nut milk when compared with those in animals in the control group. The increase was significant (p<0.05) at all instances except in the PCV of animals treated with 1 mL/kg body weight of tiger nut milk that were not significantly different from those in control animals. However, tiger nut milk had no significant (p<0.05) effect on mean corpuscular volume (MCV) and mean corpuscular haemoglobin (MCH) when compared with those in control animals. Tiger nut milk administration was observed to increase white blood cell (WBC), lymphocyte, neutrophil and platelet in a dose-dependent manner when experimental animals were compared with those in the control group. The increase was significant (p<0.05) at all instances except in the lymphocyte and neutrophil of animals treated with 1 mL/kg body weight of tiger nut milk that were not significantly (p<0.05) different from those in control animals.

Conclusion: Results of this present study indicate that tiger nut possesses haematopoietic properties and can be used to boost blood levels especially in anaemia patients. Furthermore, the plant possessed the ability to boost the immune system and thus defend the body against xenobiotics.

Haematopoiesis, immune system, tiger nut, stress-resistance

Article Details

How to Cite
Airaodion, A. I., & Ogbuagu, E. O. (2020). Consumption of Tiger Nut (Cyperus esculentus L.) Improves Haematopoiesis in Wistar Rats. International Journal of Research and Reports in Hematology, 3(1), 13-19. Retrieved from
Original Research Article


Morrison SJ, Weissman IL. The long-term repopulating subset of hematopoietic stem cells is deterministic and isolable by phenotype. Immunity. 1994;1(8):661–673.

Birbrair, Alexander, Frenette Paul S. Niche heterogeneity in the bone marrow. Annals of the New York Academy of Sciences. 2016;1370(1):82–96.

Airaodion AI, Ogbuagu EO, Ekenjoku JA, Ogbuagu U, Airaodion EO. Haematopoietic potential of ethanolic leaf extract of Talinum triangulare in Wistar Rats. Asian Journal of Research in Biochemistry. 2019; 5(2):1-7.

Nelson RG. Diabetic renal disease in transitional and disadvantaged populations. Nephrology. 2001;6:9–17.

Sánchez-Zapata E, Fernández-López J, Pérez-Alvarez AJ. Tiger Nut (Cyperus esculentus) Commercialization: Health Aspects, Composition, Properties, and Food Applications. Comprehensive Reviews in Food Science and Food Safety. 2012;11(4):366–377.

Pascual-Seva N, San-Bautista A, Galarza, SL, Maroto JV, Pascual B. Yield and irrigation water use efficiency for Ridge - and Bed - cultivated Chufa (Cyperus Esculentus L. var. Sativus Boeck). Acta Hort. 2012;936:125-132.

Ogbuagu EO, Airaodion AI. Tiger nut (Cyperus esculentus L.) boosts fertility in male Wistar rats. Asian Research Journal of Gynaecology and Obstetrics. 2020;3(3):8-18.

Zhang HY, Hanna MA, Ali Y, Nan L. Yellow nut-sedge tuber oil as a fuel. Industrial Crops and Products. 1996;5(3):177–181.

Airaodion AI, Ekenjoku JA, Ogbuagu EO, Okoroukwu VN, Ogbuagu U. Hematopoietic propensity of ethanolic leaf extract of Colocasia esculenta Linn. in Wistar rats. American Journal of Medical and Pharmacy Research. 2019;1(3):1-8.

Jain NC. Schalm’s Veterinary Haematology 4th Ed. Lea and Fabiger, Philadelphia; 1986.

Dacie JV, Lewis SM. Practical haematology, 7th Edition ELBS with Churchill Livingston, England. 1991;37-85.

Mitruka BM, Rawnsley H. Clinical, biochemical and haematological references values in normal experimental animals. Masson Publishing USA Inc. 1977;53-54.

Oguwike FN, Eluke BC, Eze RI, Asika CM, Nwosu PN, Nwafor C, Onumonu C. The effects of Cyperus esculentus (Tiger nut) on haematological and biochemical profile of male hypercholesteremic subjects in Uli, Anambra State Nigeria. Greener Journal of Medical Sciences. 2017;7(4):036-041.

Polenakovic M, Sikole A. Is erythropoietin a survival factor for red blood cells? J. Am. Soc. Nephrol. 1996;7(8):1178-1182.

Oyedeji KO. Bolarinwa AF, Akintola AM. Effect of methanolic extract of Vernonia amygdalina on haematological and plasma biochemical parameters in male albino rats. J. Dental Med. Sci. 2013;3(5):64-67.

Bamishaiye EI, Muhammad NO, Bamishaiye OM. Haematological parameters of albino rats fed on tiger nuts (Cyperus esculentus) tuber oil meal-based diet. The Internet Journal of Nutrition and Wellness. 2009;10(1):1-5.

American Diabetes Association: Nutrition recommendation and principles for people with diabetes mellitus clinical practice recommendations Diabetes care. 2000;23: 543-546.

Airaodion AI, Airaodion EO, Ekenjoku JA, Ogbuagu EO, Ogbuagu U, and Adekale OA. Investigation of haemolytic properties of ethanolic leaf and seed extracts of Telfairia occidentalis in Wistar Rats. International Journal of Research and Reports in Hematology. 2019;2(5):1-7.

Hassan HA. The potential effect of tiger nut oil on some haemato-biochemical blood

indices in male albino rats. Egyptian Journal of Experimental Biology. 2007;3: 49–54.

Mayne P. Clinical chemistry in diagnosis and treatment. (Sixth edition) Oxford University Press, Inc., New York. 1994: 281-323.

Moss PP. Blood banking: Concepts and Applications WB. Saunders Co. Philadelphia; 1999;12-34.

Robins SL. Lymphnodes and spleen. In: Pathologic basis of disease W B Sounders Co., Philadelphia. 1974;1050.

Hallberg L, Björn-Rasmussen E. Measurement of iron absorption from meals contaminated with iron. American Journal Clinical Nutrition. 1981;34:2808-2815.

Airaodion AI, Ekenjoku JA, Ogbuagu EO, Ogbuagu U, Airaodion EO. Antihaemolytic effect of ethanolic leaf extract of vernonia amygdalina in wistar rats. International Journal of Bio-Science and Bio-Technology. 2019;11(7):173-178.

Chike CPR, Njoku B, Green K, Akpojotor PI, MO. Onyebuenyi MO, Numbara D. Effect of ethanolic leaf extract of Vernonia amygdalina (Bitter Leaf) extract on some of the haematological parameters in wistar rats. Journal of Complementary and Alternative Medical Research. 2018;5(1): 1-7.

Ugochukwu NH, Babady NE. Antihyperglycemic effect of aqueous and ethanolic extracts of Gongronema latifolium leaves on glucose and glycogen metabolism in livers of normal and

streptozotocin-induced diabetic rats. Life Sci. 2002;73(15):1925-1938.

Airaodion AI, Ogbuagu U, Ekenjoku JA, Ogbuagu EO. Comparative assessment of haematopoietic potential of ethanolic extract of Telfairia occidentalis and Talinum triangulare leaves in Wistar Rats. Acta Scientific Nutritional Health. 2019; 3(10):38-43.

Li Y, Xia I, Kuter DJ. Interaction of thrombopoietin with the platelet complements receptor in plasma: Binding, internalization, stability and pharmacokinetics. Brit J. Haematol 1999; 106:345.