Rare Double Heterozygous of HbD/HbG in a Nigerian: A Case Report
Published: 2018-05-17
Page: 10-15
Issue: 2018 - Volume 1 [Issue 1]
Olusogo Ebenezer Busari *
Department of Haematology, College of Medicine and Health Sciences, Afe Babalola University, Ado-Ekiti, Ekiti State, Nigeria
Taiwo Rachael Kotila
Department of Haematology, College of Medicine, University of Ibadan, Nigeria
Omotola Toyin Ojo
Department of Haematology and Blood Transfusion, Obafemi Awolowo College of Health Sciences, Olabisi Onabanjo University, Ago-Iwoye, Nigeria
*Author to whom correspondence should be addressed.
Abstract
Aim: To advocate the use of newer and improved methods towards accurate diagnosis of haemoglobinopathies
Case Presentation: A rare case of double heterozygous of HbD/G in a pregnant female Nigerian who had present to the antenatal clinic for routine Haemoglobin electrophoresis. She had previously been diagnosed as HbAS using capillary electrophoresis and HPLC techniques.
Discussion: Capillary zone electrophoretograms showed the presence of peaks in zone Hb A, Hb D, C and a small peak in Z1 zone. Bio-Rad D10 chromatogram also indicated the presence of four peaks which are identified as Hb A, Hb D, Hb G, and hybrid of HbD/HbG. A peak in Hb D zone of capillary electrophoresis was due to co-migration of Hb D and Hb G variants. The small peak in Z1 zone indicated the presence of alpha chain variant of HbG.
Conclusion: The case exemplifies the need to use more advanced methods, including DNA analysis in order to accurately diagnose haemoglobinopathies in the nation with the largest burden of sickle cell disease.
Keywords: Haemoglobinopathies, haemoglobin electrophoresis, heterozygous, high performance liquid chromatography