International Journal of Research and Reports in Hematology

Correlation of Haematological Parameters with TNF-α and Interleukin (IL-6) in Seropositive HIV Patients on Highly Active Antiretroviral Therapy (HAART) and HAART Naïve Patients

Osei Kwame Wiredu

Department of Laboratory, Bekwai Municipal Hospital, Ashanti-Bekwai, Ghana

Fred Jonathan Edzeamey *

Department of Laboratory, Bekwai Municipal Hospital, Ashanti-Bekwai, Ghana

Albert Dompreh

Department of Serology, Komfo Anokye Teaching Hospital, Kumasi, Ghana

Delphine Gborgblorvor

Department of Out-Patients’, Bekwai Municipal Hospital, Ashanti-Bekwai, Ghana

Maxwell Awuah

Department of Medical Laboratory Sciences, School of Allied Health Sciences, College of Health and Allied Sciences, University of Cape Coast, Ghana

Ansah Kofi Ako

Department of Biomedical Sciences, School of Allied Health Sciences, College of Health and Allied Sciences, University of Cape Coast, Ghana

Gideon Darko Asamoah

Department of Medical Laboratory Sciences, School of Allied Health Sciences, College of Health and Allied Sciences, University of Cape Coast, Ghana

*Author to whom correspondence should be addressed.

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Abstract

Objective: This study sought to establish the correlation between haematological parameters and inflammatory cytokines (TNF-α and IL-6) and also to compare the effects of these cytokines in HIV/AIDS patients on HAART and HAART naïve.

Place and Duration of Study: Komfo Anokye Teaching Hospital, Kumasi from June to November 2014

Methods: HIV patients’ on HAART (n=50) and HAART naïve HIV (n=50) patients and the control group (n=50) were recruited for the study. Venous blood was drawn from each of the participant into K₃ EDTA tubes. XS-500i automatic blood cell analyzer (Sysmex Corporation Co., Ltd., Japan) was used to determine the blood parameters and ELISA Assay Kit (Greenstone Swiss Company limited, China) was used to determine TNF-α and IL-6 levels.

Results: This present study observed Hgb of 14.36 ± 0.20 g/dl in control group, 12.26 ± 0.28 g/dl and 12.13 ± 0.27 g/dl in on-HAART and HAART naïve group, RBC of 5.07 ± 0.08×10⁶/μl in control, 4.20 ± 0.09×10⁶/μl and 4.226 ± 0.09×10⁶/μl in on-HAART and HAART naïve group. IL-6 levels of 17.66(13.00 - 24.10) ng/L and TNF-α levels of 318.3(123.0 - 497.9) ng/L.

Conclusion: There was a negative correlation between some haematological parameters and the inflammatory cytokines (TNF-α and IL-6). This could be the basis for the continuous reduction in the level of blood parameters which is likely to result in haematological abnormalities such as anaemia, thrombocytopenia amongst others.

Keywords: Haematological parameters, HAART, HAART Naïve, HIV patients, TNF-α, IL-6

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