International Journal of Research and Reports in Hematology

Background: Annona muricata. L has many of medicinal properties and has been used widely in traditional medicine for treatment various disorders. Carbon tetrachloride (CCl₄) is rapidly absorbed by any route of exposure in humans and animals. Once absorbed, it is widely distributed among tissues depending on exposure concentration or dose. This study was designed to find out possible role of A. muricata suppress CCl₄ toxicity through alleviating hematological parameters disturbances in adult male rabbits.

Materials and Methods: Four rabbits per group were assigned to four groups: 0 mg A. muricata and 0 mg CCl₄/kg BW (control); 100 mg of A. muricata /kg BW; 0.5 mg CCl₄/kg BW; 0.5 mg CCl₄ plus 100 mg of A. muricata /kg BW. Rabbits were orally administered the respective doses every other day for 3 weeks.

Results: Treatment with A. muricata were not caused significant effect on values of red blood cells (RBCs), packed cell volume (PCV), hemoglobin (Hb), mean cell hemoglobin (MCH) and mean cell hemoglobin concentration (MCHC). On the other hand CCl₄ was caused increase on values of RBCs, Hb, MCV, and MCHC. Only MCV was significantly decreased with A. muricata alone. The presence of A. muricata with CCl₄ returned the values of the previous parameters close to the control values. CCl₄ caused high significant increased in mean number of WBCs compared with other three groups among three weeks of experiment. However, treatment with CCl₄ was not yielded significant deference in percentage value of neutrophil or lymphocytes compared to control. The presence of A. muricata with CCl₄ returned mean number of WBCs close to control value. Current results also indicated that treatment with CCl₄ caused a significant decreased in number of PLT while A. muricata caused increased in the number of PLT compared with control group. Oral administration of CCl₄ induced profound alterations in the morphology of erythrocytes. In rabbit treated with CCl₄ the cells were showed alteration in normal shape to star shaped or sickle cells. The mentioned alterations were less pro-found in animals treated with CCl₄ plus A. muricata. The cells had nearly normal morphology and regular contour, only some erythrocytes (not normal) could be noticed.

Conclusion: Administration of A. muricata L. in combination with CCl₄ was able to minimize and alleviate the hazardous effect of CCl₄ on most of the measured parameters. So it can be concluded that the presence of A. muricata with CCl₄ counteracted the negative effects of CCl₄ on hematological parameters in male rabbit.