Antioxidative Potential of Foetal Haemoglobin in Sickle Cell Disease
Published: 2023-09-06
Page: 179-184
Issue: 2023 - Volume 6 [Issue 2]
Yusuf Ishaya Dogonzo *
Department of Biochemistry, Federal University Ndufu Alike Ikwo (FUNAI), Ebonyi State, Nigeria.
Christopher Chimaobi Onyeabor
Department of Biochemistry, Federal University Ndufu Alike Ikwo (FUNAI), Ebonyi State, Nigeria.
Chiamaka Martha Oru
Department of Biochemistry, Federal University Ndufu Alike Ikwo (FUNAI), Ebonyi State, Nigeria.
Ogochukwu Dorothy Owusi
Department of Biochemistry, Federal University Ndufu Alike Ikwo (FUNAI), Ebonyi State, Nigeria.
Richard Chukwuebuka Ozor
Department of Biochemistry, Federal University Ndufu Alike Ikwo (FUNAI), Ebonyi State, Nigeria.
Otude Ebubechi
Department of Biochemistry, Federal University Ndufu Alike Ikwo (FUNAI), Ebonyi State, Nigeria.
Onyehara Esther Chizaram
Department of Biochemistry, Federal University Ndufu Alike Ikwo (FUNAI), Ebonyi State, Nigeria.
Oparaji Blessing
Department of Biochemistry, Federal University Ndufu Alike Ikwo (FUNAI), Ebonyi State, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Background: Oxidative stress is a clinical condition in sickle cell disease (SCD) that results from increased production of reactive oxygen species (ROS). High Foetal haemoglobin (HbF) is beneficial in sickle cell disease due to its ability to resist polymerization with sickle haemoglobin. The aim of this study is to determine the anti-oxidative potential of HbF in subjects with SCD.
Methods: Whole blood was used for the determination of HbF concentration while serum was used for the assay of Glutathione peroxidase (GPx), Super-oxide dismutase (SOD), and malondialdehyde (MDA). Alkali denaturation method was used for the determination of HbF while spectrophotometric method was used to assay for the various oxidative stress markers.
Results: The concentrations of HbF and MDA were significantly higher in the case subjects compared to the normal subjects. A direct relationship was observed between GPx (r = 0.47) and SOD (r = 0.46) with HbF. However, an inverse relationship was observed between MDA with HbF (r= -0.33), GPx (r= -0.18) and SOD (r= -0.26).
Conclusion: We conclude that HbF potentially associates with the antioxidant enzymes (GPx and SOD) to counteract the oxidative effect of ROS in SCD.
Keywords: Sickle cell disease, foetal haemoglobin, oxidative stress, lipid peroxidation, glutathione peroxidise, super oxide dismutase